Characterization of Flavobacterium psychrophilum isolated from different fish species The disease ‘rainbow trout fry syndrome’ caused by the bacterium Flavobacterium psychrophilum has since the mid 80es resulted in high mortalities in rainbow trout in Danish freshwater farms as well as in aquaculture in other European countries. Our laboratory has for several years had focus on the bacteria isolated from rainbow trout and has large background knowledge of the characteri-zation 14 of these bacteria with traditional phenotypic and molecular microbiological methods. The bacterium has to a lesser extent been isolated from perch, brown trout and farmed salmon. The aim of this project will be to analyze and compare a selected group of bacteria to find out if the bacteria isolated from different fish species are related, and therefor pose a source of infection for the farmed fish and vice versa. Methods: Phenotypic (biochemical, physical and serological) and molecular typing techniques,.e.g. pulsed-field gel elektroforese (PFGE). ECTC: 30 Contact: Associate professor Inger Dalsgaard, inda@vet.dtu.dk or Senior researcher Lone Madsen, loma@vet.dtu.dk, DTU Vet, Bülowsvej 27, 1870 Frederiksberg C Additives to fish feed – how does that influence the survival of fish pathogens? Different additives to fish feed, e.g. probiotics and essential oils of different origin, are believed to strengthen the immune status of rainbow trout. Therefore it is relevant to investigate if the survival of different fish pathogens is influenced when adding e.g. probiotics. Laboratory experiments concerning growth of fish pathogens in/on media with/without additives are planned, because a positive result here is necessary before an in vivo experiment in fish will be set up. The selection of additives and methods will be done together with the candidate. Methods: Under laboratory conditions it will be investigated how the addition of several different additives (e.g. potential probiotic candidates) will influence the growth of a fish pathogen, e.g. Flavobacterium psychrophilum (the cause of rainbow trout fry syndrome) or Yersinia ruckeri (the cause of enteric red mouth disease) in liquid media. ECTS: 30 Contact: Senior researcher Lone Madsen, loma@vet.dtu.dk, or associate professor Inger Dalsgaard, inda@vet.dtu.dk, DTU Vet, Bülowsvej 27, 1870 Frederiksberg C
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