Can lung swabs replace pieces of lung tissue for swine respiratory disease diagnosis by PCR? Short description: For diagnosis of respiratory disease pathogens in swine, a diagnostic PCR package is offered by National Veterinary Institute. With this package, lung tissue samples are tested for the presence of swineinfluenza virus, Mycoplasma hyopneumonia, porcine reproductive and respiratory syndrome virus (optional) and the level of porcine circovirus type 2 is quantitated. The purpose of the project is to validate the use of lung swabs as an alternative to the analysis of pieces of lung tissue, and to compare the performance of lung swabs and pieces of tissue for diag-nosis 42 of respiratory disease. Type of project: All Detailed description: Background: Respiratory disease is a main cause of production problems in swine herds world-wide. Identi-fication of the cause of disease, including the pathogens present in the herd, is the main key to solving these problems. The “swine respiratory disease PCR diagnostic package” offered by the National Veterinary Institute to veterinarians for diagnosis of infectious respiratory pathogens in swine herds, is a tool for identification of swineinfluenza virus, Mycoplasma hyopneumonia, PCV2 and PRRSV in lung tissue samples. Recently we tested real-time PCR test of lung tissue swabs as an alternative to test of lung tissue pieces. The two kinds of sample material showed an overall good agreement, but we wish to test the performance of a new swabbing method with an expected superior performance compared to analysis of lung tissue pieces. The project: The project is designed to compare the performance of testing lung tissue samples submitted for diagnosis of respiratory disease and a lung swabbing technique. It will include purification of RNA and DNA, real-time PCR analysis and evaluation of results. The expectation is that the results from the project may result in the launch of using lung swabbing for improved diagnostic purposes. Enquiry: Senior adviser Charlotte Kristiane Hjulsager ckhj@vet.dtu.dk Section for Diagnostics and Scientific Advice, and professor Lars Erik Larsen lael@vet.dtu.dk, Section for Virology; DTU Vet, Bülowsvej 27, 1870 Frederiksberg C. ECTS: Flexible
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